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This biochemical transformation, however, comes at an energetic cost ( Specifically, the conversion process requires a significant amount of NADPH, a crucial cofactor that primarily originates from the cytoplasmic glucose-pentose phosphate pathway

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By application of its method to several biological tissues he also substantiated that GSH/GSSG ratio in blood (or RBCs) is around 400, and a similar value also occurs in most mammalian tissues [10]