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The TACT infusion regimen included 30 weekly infusions followed by 10 maintenance infusions, the latter 2 weeks to 2 months apart

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However, these methods have drawbacks that limit their ability to generate reliable specificity maps for broad specificity proteases, including low numbers of tested peptides (usually dozens, up to thousands), proteomic library bias, or the need for modified or unnatural amino acids in the cleaved substrates

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