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In Vitro Both mitragynine and 7-hydroxymitragynine have been shown to be partial agonists at the μ -opioid receptor based on inhibition of forskolin-stimulated cyclic AMP (cAMP) accumulation (Todd et al., 2020) and antagonists at the δ and κ opioid receptors using the G-protein bioluminescence resonance energy transfer functional assay (Kruegel et al., 2016)

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