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The punctate phenotype obtained with disulfiram and its derivatives in the present study might offer a more consistent in vitro explanation for their in vivo pharmacology than does purified enzymatic inhibition, given that the EC50 of inducing punctate phenotype is 134 nM, which is less than the physiologically achievable concentration, for example about 0.34 µM with 500 mg of disulfiram per day 16

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doi: 10.2165/00002018-200225090-00001