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Human cell lines and culture conditions Plasmids expressing eGFP-CAP-D3-WT or eGFP-CAP-D3-V628E under the control of the TRE3GS doxycycline-inducible promoter were transfected into U2OS cells along with pTORA14_AAVS using Lipofectamine LTX Reagent (Fisher Scientific)

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Then we dig into a listener question about eating around non-athlete friends and family, especially when the holidays turn every meal into an opportunity for unsolicited commentary