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1 H NMR (400 MHz, D 2 O) δ 8.52 (d, J = 13.6 Hz, 1H), 7.65 (d, J = 7.2 Hz, 1H), 7.50 (dd, J = 17.6, 10.2 Hz, 2H), 7.35 (d, J = 8.0 Hz, 1H), 7.15 (s, 1H), 7.02 (d, J = 11.6 Hz, 2H), 6.36 (d, J = 15.2 Hz, 1H), 4.22 (s, 15H), 3.89 (s, 16H), 3.68 (s, 600H), 3.29 (t, J = 6.4 Hz, 4H), 2.70 – 2.58 (m, 12H), 1.81 (d, J = 6.0 Hz, 3H), 1.71 (d, J = 14.4 Hz, 6H), 0.87 (d, J = 15.2 Hz, 17H)
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Additionally, the use of fluorescent dyes and mtDNA sequencing methods may require invasive procedures to load donor cells, which could affect cell viability and induce cellular stress responses [35]