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A standard curve was prepared to estimate the limit of detection for maleimide-PEG 2 -biotin label on an intact antibody and the accuracy of quantifying the total amount of free sulfhydryls from intact protein data (Supplementary Figure S1)

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Independent HPLC purity analysis of Cagrilintide from Polaris Peptides, performed by Trust Pointe Analytics on February 12, 2026