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A study involving 25 fertile sperm donors and 20 recurrent pregnancy loss (RPL) couples showed double-stranded DNA breaks analyzed by Comet assay among RPL sperm donors without any female factor.[118] A more recent study showed that there was a positive association between RSA and high SDF.[119] REACTIVE OXYGEN SPECIES, FERTILITY CAPACITY AND ASSISTED REPRODUCTION TECHNIQUES OUTCOMES Reactive oxygen species and oxidation-reduction potential (ORP) play significant roles in the fertility process and in the outcome of ART