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For the case that the derivatization is carried out after SDS-PAGE or adsorption of samples onto ELISA plate the DNP concentration is significantly lower: 0.5 and 0.05 mM DNPH, respectively

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Two days after the first intramuscular B12 injection, the patient developed an acneiform eruption characterized by monomorphic erythematous papules and pustules involving the glabella, nose, cheeks, and perioral area ( Figure 1 )