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A combination of glutaminase inhibitor 968 and PD-L1 blockade boosts the immune response against ovarian cancer

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Briefly, cultures were grown in regular BHI until mid-exponential phase (OD 600nm ~0.4) and homologized in Tris-HCl buffer, 50 mM pH 7.4 containing 10% glycerol using a glass bead beater as described (Wen et al., 2006)