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After incubation, plates were centrifuged at room temperature for 10 min at 350 g and a tetrazolium dye MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay (Merck, Cat No

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Then, the membrane was blocked with 5% skimmed milk for 1h at room temperature