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Kinetic scheme for thymidylate synthase from Escherichia coli: determination from measurements of ligand binding, primary and secondary isotope effects, and pre-steady-state catalysis Biochemistry 36 4212-4222 1997 Tequatrovirus T4, Candida albicans, Escherichia coli, Homo sapiens, Lacticaseibacillus casei, Mus musculus brenda Tong, Y

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This suggests that these hot spots might be less important in human complexes and hence could explain the higher number of MTases interacting with human TRMT112 compared to fungi