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The rate of GSH synthesis is controlled largely by the expression and catalytic activity of the first enzyme in its biosynthesis, γ-glutamylcysteine ligase, and by the availability of cysteine (Dalton et al., 2004

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while, they are 10,000–12,000 μ M in whole brain and just 0.5–2 μ M in extracellular fluids (28)