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Western blotting Cultured cells were rinsed with cold PBS and then lysed in 1x lysis buffer containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM β-glycerophosphate, 2.5 mM sodium pyrophosphate, 1 mM Na 2 -EDTA, 1 mM EGTA, 1 mM Na 3 VO 4 , 1 μg/ml leupeptin, 1% Triton X-100, and 1x Halt protease/phosphatase inhibitors

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1 Introduction Selective serotonin reuptake inhibitors (SSRIs) increase extracellular serotonin levels by inhibiting the neurotransmitter’s reuptake by synaptic cells, thereby increasing the binding and activation of postsynaptic receptors