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Samples were then blocked with 1% Bovine Serum Albumin (Sigma-Aldrich) in PB for 30 min and then washed with 1× PBS and two washes with 0.1% PBS-Tween before incubation with primary conjugated antibodies for β-tubulin antibody (Rabbit mAb (9F3) Alexa Fluor(R) 488 Conjugate, Cell Signalling, Danvers, MA, USA) and phalloidin antibody (iFluor 555 reagent, ab176756, Abcam, Cambridge, USA) for 1 h each at room temperature