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Cell culture, transfection, and treatment Human SH-SY5Y neural cells were maintained at 37°C with 5% CO 2 atmosphere in Dulbecco’s Modified Eagle’s Media (DMEM) (Gibco) supplemented with 10% (v/v) fetal bovine serum and penicillin/streptomycin

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Peptides below roughly the glomerular filtration threshold are cleared renally, which is why kidney function is a meaningful covariate for small peptides