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ROS staining: Zebrafish embryos were incubated with 20 μM DCFH-DA (Sigma-Aldrich, San Diego, CA, USA) in the dark at 28.5 °C for 1 h, washed in 1× PBS, and then imaged

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On the other hand, LY294002-treated colon cancer stem cells showed decreases in expression of Cyclin D1, which confirms our results 114