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Unfortunately, spermatozoa are unable to repair the damage induced by oxidative stress, because they lack the required cytoplasmic enzyme systems to perform the repair (79)

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Measurement of intracellular ROS levels Cells were stained using the Cellular Reactive Oxygen Species Detection Assay Kit (Deep Red Fluorescence, Abcam) according to the manufacturer’s instructions, and analyzed with a FACSCalibur flow cytometer (Becton Dickinson)