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The specific analysis of CSF samples from amnestic MCI and AD subjects identified the early carbonylation of extracellular chaperones, suggesting that dysfunction of these pathways is initiated many years before severe dementia is diagnosed and might represent a reliable biomarker for pathology onset and progression (311, 318,319)

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Hold the syringe or pen at a 90-degree angle to your skin, pinch a fold of skin and fat between your thumb and fingers, and insert the needle steadily without hesitation—hesitation or a slow insertion can cause tissue trauma and bleeding