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To record mPTP opening, the suspension of organelles was used for 2–4 h mPTP opening was investigated by spectrophotometric registration of the swelling of mitochondria isolated from the rat heart ( 2 PO 4 —3, sodium succinate—5, pH 7.4, and a decrease in the optical density in mitochondria suspension was recorded at λ = 520 nm for 15 min of the mitochondria swelling

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