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After TMB substrate solution is added, only those wells that contain Human ICAM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

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BSO might deplete cysteine in vivo even though the mechanism of BSO action (i.e., inhibition of γ-GCS) might lead one to predict that BSO would cause a transient accumulation of cysteine (Standeven and Wetterhahn, 1991)