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Amount:3 gram

Following blocking with 5% nonfat milk (Amersham ECL blocking agent, GE Healthcare) at room temperature for 1 hr, membranes were incubated with primary antibodies at 4 °C overnight and then incubated with horseradish peroxidase (HRP) conjugated secondary antibodies (anti-mouse 1:200,000, ab6789, Abcam