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A researcher adding native IGF-1 to a serum-containing medium is therefore adding something whose free fraction is set by the binding-protein content of that particular batch of serum rather than by the amount weighed out

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Furthermore, it has been shown to be efficacious in other ulcerative colitis models, including models induced by TNBS, cysteamine, surgery, NSAIDs [73,74,75,76,77,78,79,80] or major vessel occlusion [15,16,17,18,19,20,21,22,23]