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The bound protein was eluted using 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 0.01% (w/v) LMNG and 0.0006% (w/v) CHS, 1 μM peptide, and 5 mM EGTA and 0.1 mg/mL Flag peptide