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Mass Spectrometry Analysis of Histone Modifications The cell pellet was resuspended in nuclear isolation buffer [15mM Tris-HCl (pH 7.5), 60mM KCl, 15mM NaCl, 5mM MgCl 2 , 1mM CaCl 2 , 250mM Sucrose, 1mM DTT, 1:100 Halt Protease Inhibitor Cocktail (Thermo Scientific, 78430), and 10mM sodium butyrate]