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Pooled cells in vials were thawed, counted and seeded into wells of six-well plates in RPMI-1640 without phenol red (Gibco, 11835030) supplemented with 10% FBS (Sigma-Aldrich, F7524) at a seeding density of 375,000 cells per well

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A phase I/II study of the safety and efficacy of telaglenastat (CB-839) in combination with nivolumab in patients with metastatic melanoma, renal cell carcinoma, and non-small-cell lung cancer