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The next day, cells were washed three times in 0.1% Tween 20 in PBS and incubated with secondary antibodies conjugated with Alexa Fluor 488, TRITC, or Alexa Fluor 647 (diluted 1:400 in 5% NDS) for 2 h at room temperature

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The FASCINATE-1 trial (NCT03938246) evaluated TVB-2640, a first-in-class oral FASN inhibitor, in patients with NAFLD and stage 1–3 fibrosis [57]