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Aisha Abdali - Head of Product Marketing | AI, GTM Strategy, Platform & Monetization | Ex-Spotify, Dropbox | LinkedIn
Nutrient stress triggers a phosphorylation-lactylation cascade: ULK1 phosphorylates LDHA (S196), boosting lactate production and subsequent KAT5/TIP60-mediated VPS34 lactylation (K356/K781), which enhances VPS34 activity to promote autophagosome-lysosome fusion and sustain autophagic flux [51, 52]
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and mitochondrial O 2 - , by seeding cells in glass bottom culture dishes, staining with MitoSOX™ Red (Life Technologies, Grand Island, NY, US) and examining them under a Zeiss LSM510 Meta NLO confocal microscope (Carl Zeiss Microscopy, LLC, Thornwood, NY, USA)