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It is a reason to recognize that the risk-benefit calculation cannot be made with current data, and anyone describing the risk profile with confidence in either direction is overreaching the evidence

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Real-time PCR was performed in parallel in 25 μl reactions containing 12.5 μl 2 × QuantiTect SYBR Green PCR master mix (Qiagen, Venlo, The Netherlands) and 2.5 μl 10 × QuantiTect Primer Assay (Qiagen)