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During IVM, FLI medium activates key signaling pathways, including the phosphorylation of protein kinase B (AKT), mitogen-activated protein kinase 1/3 (MAPK1/3), signal transducer and activator of transcription 3 (STAT3), and the mammalian target of rapamycin (mTOR) downstream target ribosomal protein S6 kinase B1 (RPS6KB1) ( Other interventions further underscore the importance of metabolic regulation in oocyte development

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You can then move the digit slowly to see if the needle is hitting bone proximal or distal to the joint