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Karyopyknotic cells in the affected brain areas were analyzed (0–4) (a score of 0 indicates no change), including the cerebral cortex (NTP-7, Level 3), cerebellar cortex (NTP-7, Level 6), hippocampus, thalamus, and hypothalamus (NTP-7, Level 3), with scoring categorized as follows: a few karyopyknotic neuronal cells (≤20%) (score of 1)