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David Sinclair's lab at Harvard demonstrated that NAD+ restoration via NMN supplementation reversed age-related muscle degeneration and improved mitochondrial function in aged mice (Gomes et al., Cell, 2013)

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Cell culture HeLa cells were grown in DMEM supplemented with fetal bovine serum (FBS, 10%) and penicillin (100 U ml −1 )/streptomycin (100 µg ml −1 )/fungizone (0.25 µg ml −1 ) at 37 °C in a 5% CO 2 environment