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Lentiviral particles were generated by transfecting the pLKO.1 mCherry plasmid into 293 T cells using a VSV-G envelope and psPAX2 packaging plasmid

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However, NKCC2A KO islets also showed increased expression of KCC2, i.e., a furosemide-sensitive and constitutively active K + Cl − cotransporter (Payne, 1997

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