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For nuclei extraction, the cells were incubated with 0.1% (v/v) digitonin (Promega, USA) for 3 min at 4 °C and centrifuged for 10 min at 500 x g at 4 °C with 1 mL ATAC-RSB, containing 0.1% (v/v) Tween-20

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We calculated the ratio of the emission (405/488 nm) and plotted it against time or concentration of antimalarial drugs