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The absorbance of the mixture was measured at 517 nm using a spectrophotometer

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G6PD deficiency and sickle cell trait/disease have similar geographic distributions and can occur together in up to 5% of men of African descent, 8 so it is reasonable to consider testing athletes with sickle cell for G6PD deficiency if they present with unexplained rhabdomyolysis or for those who may be exposed to offending medications such as uric acid–lowering medications or chemotherapy agents