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Decreased resting energy expenditure in patients with Duchenne muscular dystrophy

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There are some limitations of the FRAP assay, such as the redox potential of the Fe 2+ /Fe 3+ pair that can induce a false Fe 3+ reduction when testing compounds with a lower redox potential, the rapid time of reaction which considers that redox reactions are fast, but is not always the case, and the hydrophilic environment of the reaction preventing the hydrophobic compounds from being efficiently tested [149]