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In human PAECs, hypoxia promotes the expression of HIF-1α by upregulating the IL-33/ST2 axis, which then increases the expression of VEGFA and VEGFR-2, leading to enhanced adhesion and angiogenesis

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Bacterial suspensions were cotreated with 10 nM PI and specified agents, followed by spectrofluorometric quantification of fluorescence intensity using standardized parameters (λex = 535 nm, λem = 615 nm) with a microplate reader