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With a free energy change of –52.6 kcal/mole, it is clear that NADH oxidation has the potential for driving the synthesis of a number of molecules of ATP since the standard free energy for the reaction below is +7.3kcal/mole: ADP + P i → ATP Classically, the description of ATP synthesis through oxidation of reduced electron carriers indicated three moles of ATP could be generated for every mole of NADH and two moles for every mole of FADH 2